Fecha/hora: 16/05/2024 07:25:15

Biblioteca(s): 	INIA La Estanzuela.	
Fecha corriente: 	19/06/2019	
Fecha de la última actualización: 	05/09/2022	
Autoria: 	ALMEIDA, D.S.; PAZ, L.N.; DE OLIVEIRA, D.S.; RISTOW, P.; HAMOND, C.; COSTA, F.; PORTELA, R.W.; ESTRELA-LIMA, A.; PINNA, M.H.	
Afiliación: 	Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Instituto Gonçalo Moniz, Ministério da Saúde, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil Silva, D.N. // Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Laboratory of Bacteriology and Health, Institute of Biology, Federal University of Bahia, Salvador, Bahia, Brazil.; CAMILA HAMOND, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Saude Coletiva, Federal University of Bahia, Salvador, Brazil.; Instituto de Ciências da Saúde, Federal University of Bahia, Salvador, Brazil.; Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.	
Título: 	Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse.	
Año de publicación: 	2019	
Fuente/Imprenta: 	PloS One, 2019, Volume 14, Issue 5, Page e0217391. [OPEN ACCESS.].Doi: https://doi.org/10.1371/journal.pone.0217391	
DOI: 	10.1371/journal.pone.0217391	
Idioma: 	Inglés	
Notas: 	Article history: Received: August 14, 2018 / Accepted: May 11, 2019 / Published: May 23, 2019.  This study was funded by the Foundation
for Research Support of the State of Bahia, Brazil (http://www.fapesb.ba.gov.br/), Public Notice no.025/2010 under Term of Grant PPP 0034/2011,coordinated by Melissa Hanzen Pinna. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.	
Contenido: 	ABSTRACT.
The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected sheep confirmed by PCR characterizes renal colonization in a species with the presence of histological changes compatible with leptospirosis.	
Palabras-Clave: 	DISEASES OF SHEEP; LEPTOSPIRES; PLATAFORMA EN SALUD ANIMAL.	
Thesagro: 	ENFERMEDADES DE LOS ANIMALES; LEPTOSPIROSIS.	
Categoría del tema: 	E16 Enfermedades de los animales	
URL: 	http://www.ainfo.inia.uy/digital/bitstream/item/16705/1/pone.0217391.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6532964/pdf/pone.0217391.pdf	
Registro original: 	INIA La Estanzuela (LE)	
Ejemplares: 	LE - (102904-1) - PXI - AP - DD - PP/PloS One/2019 -  -  - DIS